Striker Ryan Babel has been ruled out of the Euro 2008 tournament starting next week after sustaining an injury in training, Dutch football officials said on Saturday.
The Liverpool forward is thought to have ruptured his Achilles tendon.
Coach Marco van Basten has yet to name his replacement.
Holland who won the tournament 20 years ago open their Euro 2008 campaign against Italy on June 9 and will play France four days later.